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| WBPicture0000010080 | Description | Figure 1. CeTLF Protein Is Nuclear and Present in All Cells throughout Embryogenesis. (A) Schematic representation of the Caenorhabditis elegans (Ce) tlf-1 genomic structure. The cDNA (starting with the SL1 splice leader) was aligned to the genomic sequences. Open boxes indicate the exons, and gray regions indicate the exons that encode the two direct repeats of CeTLF (Dantonel et al. 1999).(B) Homology of CeTLF amino acid sequence with the C. elegans TBP (CeTBP). The percentage of similarity between the core domains (shown in gray) is indicated. 'n.s.' denotes nonsignificant.Wild-type embryos (WT) were stained with polyclonal antibodies against CeTLF-1 (D, F, and H) and the DNA-specific dye DAPI (C, E, and G).(C and D) Two-cell stage embryo.(E and F) Mid-embryogenesis embryo (end of proliferation). All cells express CeTLF, and the signal is nuclear.(G and H) Mid-embryogenesis embryos that were coincubated with polyclonal antibodies against CeTLF and an excess of competitor peptide used to generate the anti-CeTLF antibody. The nuclear signal is abolished.(I and J) The nuclear signal is eliminated in tlf-1(RNAi) embryos. In this and subsequent figures, embryos are oriented with anterior to the left and dorsal up; the scale bar indicates 10 um.(K) Forty WT or tlf-1(RNAi) embryos were collected at different time points after injections (as indicated) and allowed to reach their terminal phenotypes. The four different time points correspond to the four time points shown in Table 1. Embryos were boiled directly in 10 ul of SDS-PAGE sample buffer, and proteins were analyzed by Western blot using antibodies raised against CeTLF, CeTBP, and the CTD of Pol II. The hyperphosphorylated (IIO) and the hypophosphorylated (IIA) forms of the large subunit of Pol II are labeled. | |||
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| Name | F1.jpg | ||||
| Crop | Crop_picture | WBPicture0000010081 | |||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2000 | ||||
| Article_URL | DOI | id | 10.1016/S1097-2765(00)00069-1 | ||
| Journal_URL | MolecularCell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00004352 |
