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WBPicture0000009900 Description: Figure 4. eff-1 Expression in Fusing Cells and Models of EFF-1 Structure and Function in Cell Fusion. Expression of eff-1p::gfp in wild-type embryos and worms.(A-C) Time course of expression in a single embryo.(A) An approximately 500-cell embryo (250 min) showing first expression of GFP in epidermal precursors.(B) Ventral view of enclosed embryo (340 min) with GFP in ventral (v) and dorsal (d) epidermal cells.(C) Lateral view at 1.5-fold stage (430 min), with GFP throughout hyp6 and hyp7 syncytia and in tail spike cells (ts).(D) L2 showing GFP in lateral and dorsal hyp7, but no expression in the seam cells (se). A seam daughter cell (se-hyp7) expresses GFP intensely before fusing to hyp7.(E) Head of adult hermaphrodite, with GFP in pharyngeal metacorpus (ph), dorsal hyp7, amphid sheath cells (as), and neuronal axons (ax) projecting from the nerve ring (nr).(F) Midsection of mid-L3 with GFP in lateral (top) and ventral (bottom) hyp7. Dividing unfused VPCs remain unlabeled.(G) Vulval morphogenesis in mid-L4. Subset of vulval cells express GFP (green overlaid on DIC). Three-dimensional reconstruction of two tetranucleated toroids (inset, rotated 90 degrees) shows lumen (open arrowhead) forming through centers of toroids (arrow and filled arrowhead).(H) Midsection of gravid hermaphrodite showing GFP in a uterus containing embryos.(I) Copulatory bursa of adult male tail with GFP in fan and rays.Asterisks show cells not known to participate in cell fusion. The scale bars represent 10 μm.(J) Model of EFF-1 primary structure, indicating known point mutations, putative functional domains, and sites of possible modification.(K) Model of EFF-1 action in an early stage of a cell fusion event (Mohler et al., 1998). Two cells lie in close contact. EFF-1 is required for formation of an initial opening through the double bilayer at the adherens junction, and rapid diffusion of cytoplasm between cells. Anterior-posterior (A-P), left-right (L-R), and apical-basal (Ap-Ba) axes are shown.
  Name: F4.jpg
  Crop: Crop_picture: WBPicture0000009901
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2002
    Article_URL: DOI id 10.1016/S1534-5807(02)00129-6
    Journal_URL: DevelopmentalCell
    Publisher_URL: Elsevier
  Reference: WBPaper00005122