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| WBPicture0000009736 | Description | Figure 1. Expression of gpa-12 in C. elegans as Detected by Using a Translational GFP Gene Fusion. (A and B) pRP2018 contains the full-length wild-type gpa-12 sequence fused to gfp. (A) The head region of an L2-staged larva showing expression of GPA-12 in the pharyngeal muscle cells (ph). (B) L1 larva showing expression in the cytoplasm and nuclei of the hyp7 syncytium (sy), which comprises the main body hypodermis, and in additional cells, which are presumably the P cells (precursors of motorneurons in the ventral nerve cord). No expression is detected in hypodermal seam cells (V1-V6 cells). The nuclear localization of the gpa-12::gfp reporter construct (pRP2018) may reflect the endogenous GPA-12 expression, since the gfp-reporter vector used does not contain a nuclear localization signal. We observed an identical expression pattern of GPA-12 by using another gpa-12::gfp reporter construct (pRP2015; see the Supplemental Experimental Procedures), including nuclear localization. | |||
|---|---|---|---|---|---|
| Name | F1.jpg | ||||
| Depict | Expr_pattern | Expr2374 | |||
| Anatomy | WBbt:0005451 | ||||
| WBbt:0005734 | |||||
| Cellular_component | GO:0005634 | ||||
| GO:0005737 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1016/S0960-9822(03)00164-7 | ||
| Journal_URL | CurrentBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00005783 |
