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WBPicture0000009720 Description: Figure 5. Similar Axon Navigation Defects in ina-1- and mig-15-Deficient Animals. (A) A schematic diagram of C. elegans GABAergic neurons: the name of all GABAergic neurons are indicated below the cell bodies (white circles); DC, dorsal cord; CO, commissure; VC, ventral cord. Anterior is oriented toward the left, and dorsal is oriented upward. Note that most commissures are on the right side of the animal (curved lines pointing to the tail), while two in the head are on the left side (curved lines pointing to the head).(B) GFP autofluorescence of a wild-type control animal carrying the unc-47::gfp transgene expressed in all GABAergic neurons.(C-J) Commissures from a (C) mig-15(RNAi) animal, a (D) mig-15(rh148) mutant, an (E) ina-1(gm144) mutant, an (F) ina-1(gm119); mig-15(rh148) double mutant, an (G) ina-1(gm144); mig-15(RNAi) mutant, an (H and I) animal carrying a transgene expressing mig-15 dsRNA and pat-3 dsRNA, respectively, under the control of the unc-25 GABAergic promoter, and an (J) ina-1(gm144) mutant after stable mig-15 RNAi under the control of the unc-25 promoter. All animals carry the unc-47::gfp construct. Note that most animals have meandering commissures and/or prematurely branched commissures (arrows) that generally lead to a disruption of the dorsal cord (arrowheads), and that the defects are significantly more severe when animals are defective for both ina-1 and mig-15.(K) Upper panel, expression of mig15::gfp in neurons of an L2 larva in the area of P7.p-P8.p cells (ventral side is up); lower panel, DIC (differential interference contrast) picture of the same animal (only the ventral cord is shown).(L) Expression of mig-15::gfp in the dorsal cord (DC) in an L3 animal.(M) Expression of mig-15::gfp in the ventral cord (VC), body wall (BM), vulval (VM) muscles, and vulva (V) of a young adult. In panels (H)-(M), the animal appears to be twisted because of the dominant marker rol-6(su1006) used as a cotransformation marker. The scale bar represents 30 μm in (B)-(L) and (M) and 6 μm in (K).
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  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2002
    Article_URL: DOI id 10.1016/S0960-9822(02)00764-9
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00005218