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| WBPicture0000009702 | Description | Characterization of the nhr-8 spatial expression pattern. (a,b) The nhr-8::GFP was constructed by a two-step PCR cloning strategy and contains 6.8 kb of upstream genomic sequence, the nhr-8 transcription and translation start sites, and 49 nhr-8 codons fused in frame to GFP-coding sequences. A total of five independently isolated nhr-8::GFP fusion plasmids were used to generate transgenic nematode strains. All transgenic strains exhibited identical GFP activity. (a) DIC micrograph of a transgenic larva (arrows mark the anterior/posterior extent of the intestine of the transgenic larva) and a wild-type larva (indicated by an arrowhead). (b) Epifluoresence micrograph of the animals in (a), revealing fusion protein expression only in the transgenic animal. | |||
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| Name | F2_A.jpg | ||||
| Crop | Cropped_from | WBPicture0000009701 | |||
| Depict | Expr_pattern | Expr1351 | |||
| Anatomy | WBbt:0005792 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2001 | ||||
| Article_URL | DOI | id | 10.1016/S0960-9822(01)00236-6 | ||
| Journal_URL | CurrentBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00004723 |
