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WBPicture0000009690 | Description | (a) Expression of nos-3 mRNA. Polyadenylated C. elegans mRNA was separated through a 1% agarose glyoxal gel, transferred to a filter and probed with a nos-3 cDNA. Lane 1, wild-type adults, which possess approximately 2,000 germ cells; lane 2, glp-1(q224) mutant adults, which possess no more than eight germ cells; lane 3, mixture of developmental stages (from first larval stage through adults with embryos); lanes 4-9, embryos (E), the four larval stages (L1-L4) and adults (A), respectively. In lane 2, note that very little nos-3 mRNA is detectable; the small amount remaining may represent somatic expression. To verify equal loading of lanes, the blot was probed for expression of elongation factor-1 alpha (eft-3). | |||
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Name | F3_A.jpg | ||||
Crop | Cropped_from | WBPicture0000009689 | |||
Depict | Expr_pattern | Expr1202 | |||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
Publication_year | 1999 | ||||
Article_URL | DOI | id | 10.1016/S0960-9822(99)80449-7 | ||
Journal_URL | CurrentBiology | ||||
Publisher_URL | Elsevier | ||||
Reference | WBPaper00003692 |