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| WBPicture0000009646 | Description | Figure 4. Localization of TBH-1 and TDC-1. (A and B) Western blot analysis of total protein of (1) wild-type, (2) tdc-1(n3419), (3) tdc-1(n3420), (4) tdc-1(n3421), (5) tbh-1(n3247), and (6) tbh-1(n3722) animals with (A) TBH-1-antibodies and (B) TDC-1-antibodies. (A) In the tbh-1(n3722) mutants, a 45 kDa band is detected, in agreement with the predicted size of the protein that results from the in-frame deletion of the n3722 allele. (B) The weak 60 kDa band from tdc-1(n3419) mutants correlates with the predicted size of the protein of this in-frame deletion allele. (C and D) Whole-mount staining with TBH-1-antibodies. TBH-1 is expressed in the (C) RIC interneurons, where it is mainly localized to synaptic specializations and (D) gonadal sheath cells. (E and F) Whole-mount staining with TDC-1-antisera. TDC-1 is expressed in (E) the UV1 cells in the late L4 larva and the (F) gonadal sheath cell projections between the oocytes in adults. (G-I) Double staining of a tbh-1::gfp transgenic animal with (G) TDC-1-antisera and (H) mouse monoclonal GFP-antibodies. TDC-1 is expressed in the RIM head neurons and the RIC interneurons. (I) Merged. Anterior is on the left (C-I). Scale bar, 10 μm. | |||
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| Name | F4.jpg | ||||
| Crop | Crop_picture | WBPicture0000009647 | |||
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| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2005 | ||||
| Article_URL | DOI | id | 10.1016/j.neuron.2005.02.024 | ||
| Journal_URL | Neuron | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00025096 |
