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| WBPicture0000009632 | Description | Figure 5. Focus of Action of HS-Modifying Enzymes. (A-C) Expression of hse-5, hst-2, and hst-6 reporter gene constructs. Fusions contain all the 5' upstream region to the preceding gene. Expression of hse-5::gfp in comma stage embryos is strong in the hypodermis and intestine and weaker in the head region (A). hst-2::gfp and hst-6::gfp are ubiquitously expressed in late gastrulation (left panel in B and C) and comma stage embryos (middle panel in B and C). Expression levels of hst-2::gfp is stronger in intestinal cells than in surrounding tissue. Expression of all three reporter constructs perdures in a more tissue-restricted manner in late embryos, larval, and adult stages (right panels each). hse-5::gfp expression is primarily seen in the hypodermis (A) and the intestine (not shown; the animal shown for hse-5 exhibits expression exclusively in the hypodermis due to mosaicism). hst-2::gfp shows a wider expression pattern in adults, e.g., in the hypodermis (white arrowheads, hypodermal ridge; black arrowheads, lateral hypodermis) and a restricted set of ventral motorneurons (white arrows) (B), whereas expression for hst-6::gfp is primarily observed in the nervous system (C).(D) Rescue of the PVQ axon crossover phenotype with different expression constructs. The number of extrachromosomal lines for a particular construct that rescue the defect is shown. Promoters are not to scale and comprise 216 nt for dpy-7 and 631 nt for hst-6.a3 out of 6 rescuing lines showed partial rescue (50%-75%) of the defects.b2 out of 3 rescuing lines showed partial rescue (50%-75%) of the defects. | |||
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| Name | F5.jpg | ||||
| Crop | Crop_picture | WBPicture0000009633 | |||
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| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2004 | ||||
| Article_URL | DOI | id | 10.1016/S0896-6273(04)00084-4 | ||
| Journal_URL | Neuron | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00006471 |
