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| WBPicture0000009606 | Description | Figure 2. N-terminal GFP::NMR-1 Fusion Protein Appears Localized at Synapses. Approximately 5 kb of nmr-1 5' untranslated sequence fused to GFP was used to show expression of nmr-1 in a small subset of neurons.(A and B) A confocal image (A) and schematic representation (B) of a transgenic worm that expressed the nmr-1::GFP promoter fusion. Anterior regions are shown on top and posterior regions on the bottom.(C) A transgenic worm that expressed an N-terminal GFP::NMR-1 fusion protein where GFP was fused immediately after the signal sequence. A punctate pattern of expression was observed in the neural processes of the nerve ring (arrowheads) and ventral cord (arrows), suggesting that the fusion protein was localized to synapses.(D) A transgenic worm that expressed a C-terminal NMR-1::GFP fusion protein. Although difficult to see, no puncta were observed in the processes of either the nerve ring (arrowhead) or ventral cord (arrow). The diffuse distribution of NMR-1::GFP was most evident in the process of the AVD interneuron (asterisk). | |||
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| Name | F2.jpg | ||||
| Remark | A C-terminal NMR-1::GFP fusion protein was most evident in the process of the AVD interneuron. | ||||
| Depict | Expr_pattern | Expr2402 | |||
| Anatomy | WBbt:0003850 | ||||
| WBbt:0005300 | |||||
| WBbt:0005835 | |||||
| WBbt:0005840 | |||||
| WBbt:0005842 | |||||
| WBbt:0006749 | |||||
| WBbt:0006818 | |||||
| WBbt:0006819 | |||||
| Cellular_component | GO:0045202 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2001 | ||||
| Article_URL | DOI | id | 10.1016/S0896-6273(01)00394-4 | ||
| Journal_URL | Neuron | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00004836 |
