WormBase Tree Display for Picture: WBPicture0000009604

WBPicture0000009604 Description: (I) A full-length SAD-1::GFP fusion protein was expressed in the nervous system using the unc-115 promoter. GFP was inserted in frame at amino acid 566 of the SAD-1 protein. Sequences 5' to GFP were from the sad-1 cDNA, and sequences 3' were from the sad-1 genomic clone. Shading of domain structure as in Fig. 4(B), GFP in green. (J) SAD-1::GFP localized to puncta within the axons of the nerve ring (thick arrow) and ventral (white arrowhead) and dorsal (yellow arrowhead) nerve cords but was faint or absent in the dendrites (thin arrow) and synapse-poor commissures. Additionally, SAD-1::GFP was observed in cell bodies, where it was excluded from the nucleus (asterisk). These animals were fixed with formaldehyde to eliminate gut autofluorescence. (K) A higher magnification of the ventral cord in animals expressing punc-115SAD-1::GFP (green) and costained with anti-SYD-2 (red) antibodies. Little colocalization of SAD-1::GFP and SYD-2 was evident (e.g., arrowhead). (L) A higher magnification of the dorsal cord in animals overexpressing SAD-1 from its own promoter and costained with anti-SAD-1 (red) and anti-SNT-1 (green) antibodies. Many SAD-1 and SNT-1 puncta overlapped (yellow color/white arrowheads) or were adjacent (red arrowheads). Most SNT-1 puncta were associated with SAD-1, but some SAD-1 puncta were not associated with SNT-1 (yellow arrowheads).
  Name: Fig5_C.jpg
  Crop: Cropped_from: WBPicture0000009601
  Depict: Expr_pattern: Expr814
    Anatomy: WBbt:0005829
      WBbt:0006749
      WBbt:0006750
    Cellular_component: GO:0030424
      GO:0043025
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2001
    Article_URL: DOI id 10.1016/S0896-6273(01)00184-2
    Journal_URL: Neuron
    Publisher_URL: Elsevier
  Reference: WBPaper00004523