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| WBPicture0000009584 | Description | Figure 3. Expression of an egl-36::gfp Fusion. Each panel is a merged epifluorescence image of GFP and a Nomarski image. In the top part, white arrows indicate GFP expressed in the vulval muscles, and the black arrowhead indicates the vulval opening. The vulval muscles run diagonally from the lips of the vulva to the lateral body wall, and only one segment of the muscles is in the focal plane of this photograph. In the middle part, the white arrow indicates GFP expressed in the neuron DVB, and the black arrowhead indicates the opening of the rectum. In this image, the pattern most commonly seen in neuronal expression of this fusion is apparent: a ring of fluorescence at the perimeter of the cell body, presumably indicative of general cell-surface localization, and a brighter region near one edge of the cell body. In the bottom part, the white arrows indicate GFP expressed in two lateral ganglion neurons that were tentatively identified as RIAL (upper and fainter) and AVEL (lower). The two black arrowheads indicate GFP expressed in two pharyngeal neurons M3 (left, barely visible) and I6 (right). The bright spot near the right of the image is an out of focus image of GFP expressed in ADEL. The relative intensities of fluorescence from the cells in these images are approximately characteristic of the cells based on direct observation. Transgenic worms phenocopy egl-36(gf) mutants for both Egl and Exp phenotypes (Figure 3 and data not shown). This could result from overexpression of the fusion gene in transgenic lines, in which multiple copies of injected DNA are organized in tandem into an extrachromosomal array (Mello et al. 1991). Alternatively, the insertion of GFP into the C-terminus could alter channel function, causing a dominant phenotype. | |||
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| Name | F3.jpg | ||||
| Depict | Expr_pattern | Expr1466 | |||
| Anatomy | WBbt:0003754 | ||||
| WBbt:0003864 | |||||
| WBbt:0003960 | |||||
| WBbt:0004009 | |||||
| WBbt:0004739 | |||||
| WBbt:0004822 | |||||
| WBbt:0005821 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1997 | ||||
| Article_URL | DOI | id | 10.1016/S0896-6273(00)80355-4 | ||
| Journal_URL | Neuron | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00002851 |
