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| WBPicture0000009567 | Description | Figure 2. Confocal Micrographs of UNC-6::HA Staining in Early Embryos. Anterior is shown on the left. Scale bars, 5 μm. (A) Ventral horizontal plane showing netrin expression during neurulation when the ventral epidermoblasts P1/2-P11/12 spread ventrally to enclose the embryo (230-290 min). For landmarks, these embryos were costained with mAb OIC1D4, which recognizes cytoplasmic granules (asterisks) in germline cells positioned on the ventral midline. (left panel) About 250 min: in late gastrula, the ventral epidermoblasts form two groups of six cells on either side of the embryo. In this plane, bright cytoplasmic staining is visible within all six cells of the left group. (right panel) About 280 min: midway through neurulation, the ventral epidermoblasts have formed into two regular rows converging toward the midline. (B-D) Midsagittal planes showing netrin expression in early neurulae. (left panels) UNC-6::HA is shown in yellow. (right panels) Anaglyph with cell nuclei shown in red (propidium iodide stain). (B) About 340 min: inner labial (ILsh) and ventral cephalic sheath cells (CEPshV) are visible in the head. Neuroblast I5 is visible within the developing pharynx. Ventral epidermoblasts are still visible in the body. (C) About 430 min: the sheaths have moved anteriorly, leaving a scaffold of netrin-labeled processes underlying the developing nerve ring and labial nerves. Neuron PVT is now visible in the preanal region, while the ventral epidermoblasts are no longer detectable in the body. (D) About 415 min: within the terminal bulb of the pharynx, neuron I5 is visible just ventral to the adherens junctions that anticipate the pharyngeal lumen. For landmarks, this embryo was costained with mAb MH27, which recognizes adherens junctions in the pharynx and other epithelia. | |||
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| Name | F2.jpg | ||||
| Depict | Expr_pattern | Expr1590 | |||
| Anatomy | WBbt:0004070 | ||||
| WBbt:0004527 | |||||
| WBbt:0004529 | |||||
| WBbt:0004740 | |||||
| WBbt:0004923 | |||||
| WBbt:0004925 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1996 | ||||
| Article_URL | DOI | id | 10.1016/S0896-6273(00)80021-5 | ||
| Journal_URL | Neuron | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00002378 |
