WormBase Tree Display for Picture: WBPicture0000009542

WBPicture0000009542 Description: Figure 4. SAS-4 Is Incorporated during Centriole Duplication and Remains Stably Associated Thereafter. Wild-type (N2) males were mated with hermaphrodites expressing GFP-SAS-4. Embryos were fixed at various times after fertilization and stained for microtubules (MTs) and DNA (red and blue in left images) and with antibodies to SAS-4 to detect both the labeled and unlabeled protein (total SAS-4) and to GFP to detect only the GFP-SAS-4 fusion (GFP-SAS-4). Centrosomes are shown in the insets (magnified 3). A schematic of our interpretation of the results is shown on the right.(A) In recently fertilized embryos, SAS-4 localizes to the centriole pair associated with the sperm nucleus. There is no staining in the GFP channel.(B) By prophase of the first mitotic division, GFP-SAS-4 has been incorporated into both centrosomes.(C) GFP-SAS-4 is also detected in a single focus in the center of each centrosome at metaphase.(D) When the centrosomes split in telophase, each daughter centrosome inherits one centriole. Both centrosomes have a focus of SAS-4 staining. However, only one of the centrosomes, likely the one containing the daughter centriole synthesized in the embryo, contains GFP-SAS-4.(E) By prophase of the two-cell stage the centrioles have duplicated again. All of the centrosomes contain at least one centriole that was synthesized in the oocyte cytoplasm and all contain the GFP-SAS-4 fusion protein. Scale bar is 10 μm.
  Name: F4.jpg
  Depict: Expr_pattern: Expr2460
      Expr2461
    Cellular_component: GO:0005813
      GO:0005814
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2003
    Article_URL: DOI id 10.1016/S0092-8674(03)00117-X
    Journal_URL: Cell
    Publisher_URL: Elsevier
  Reference: WBPaper00005739