WormBase Tree Display for Picture: WBPicture0000009541

WBPicture0000009541 Description: Figure 3. SAS-4 Localizes to Centrioles Throughout the Cell Cycle. (A) Wild-type hermaphrodites were fixed and stained for DNA, gamma-tubulin, and SAS-4. SAS-4 colocalized with gamma-tubulin to centrosomes in sperm (arrow on left side of all images and insets) and to centrosomes associated with nuclei in meiotic prophase in the gonad arm (small arrows), but disappeared in oocytes (arrowheads). Insets were magnified 3.(B) Hermaphrodites expressing GFP-SAS-4 were examined by DIC (a, b) and for GFP fluorescence (a', b'). As was the case for the SAS-4 antibody staining, single punctate fluorescent foci were found in sperm and associated with meiotic nuclei in the gonad arm. Arrows in (a) and (a') indicate the location of a cluster of GFP-SAS-4 foci.(C) Wild-type embryos were fixed and stained to visualize microtubules (MTs) and DNA (green and red in left images), gamma-tubulin (middle images), and SAS-4 (right images). In recently fertilized embryos in which the oocyte nucleus was still completing meiosis (top images), SAS-4 was observed in a single punctate focus associated with the sperm pronucleus (arrows). No colocalizing gamma-tubulin staining was observed at these early time points. (C) At metaphase, two small foci of SAS-4 staining are seen in the center of the gamma-tubulin staining at each spindle pole.(D) Stills taken from a time-lapse recording of an embryo expressing GFP-SAS-4. Both DIC (a) and GFP fluorescence (a') are shown. In the DIC image, the mitotic spindle is visible as a region that excludes yolk granules. Punctate foci of GFP-SAS-4 are observed in the center of each spindle pole.(E) Higher magnification (6.4) view of the right centrosome in the metaphase image in (C). Scale bars in (A-D) are 10 μm.(F) Localization of SAS-4 by immunoelectron microscopy. Immunolabeling was found associated with centriole walls. Labeling was detected on both mother and daughter centrioles. Scale bar is 250 nm. Images in the right column show selected centrioles from the images on the left magnified 2.5.
  Name: F3.jpg
  Depict: Expr_pattern: Expr2460
      Expr2461
    Cellular_component: GO:0005813
      GO:0005814
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2003
    Article_URL: DOI id 10.1016/S0092-8674(03)00117-X
    Journal_URL: Cell
    Publisher_URL: Elsevier
  Reference: WBPaper00005739