WormBase Tree Display for Picture: WBPicture0000009532
expand all nodes | collapse all nodes | view schema
| WBPicture0000009532 | Description | Figure 4. VAB-2/EFN-1 Is Expressed in Neurons Adjacent to VAB-1-Expressing Cells. Confocal images of VAB-2/EFN-1 expression from the juIs53 [vab-2/efn-1(+)] transgene, visualized using anti-VAB-2/EFN-1 antibodies (green); epidermal adherens junctions were visualized using the MH27 monoclonal antibody (red); VAB-1::GFP expression in (D)-(F) visualized with anti-GFP antibodies (blue). Bar, 10 μm (A, B, D-H), 20 μm (C).(A and B) Ventrolateral and ventral views of early enclosure. During epidermal enclosure VAB-2/EFN-1 is expressed in many neurons in the anterior and in several in the tail. Based on double staining with the epidermal markers MH27 and LIN-26, VAB-2/EFN-1 is not expressed in epidermal cells or precursors. Leading epidermal cells (arrows) appear to migrate directly over VAB-2/EFN-1-expressing neurons.(C) Late embryo, 3-fold stage; the nerve ring (arrowed), head neurons, and dorsal and ventral nerve cords express VAB-2/EFN-1. Strong expression is also seen in the metacorpus, isthmus, and valve regions of the pharynx.(D) Triple label immunofluorescence (confocal section) showing VAB-2/EFN-1 (green), VAB-1::GFP (blue), and MH27 (red). Lateral view during early enclosure; leading edges of epidermal cells are arrowed.(E and F) VAB-2/EFN-1 and VAB-1::GFP expression after leading cells have met at the ventral midline (arrow). Ventral views; (E) is a confocal section showing only VAB-2/EFN-1 and VAB-1::GFP channels; (F) is a confocal projection of the ventral half of the embryo in all three channels. VAB-1::GFP and VAB-2/EFN-1 expressing cells form distinct, adjacent populations.(G and H) VAB-2/EFN-1 expression in vab-1(e2) mutant embryos. VAB-2/EFN-1-expressing cells become more spread out, compare brackets showing extent of VAB-2/EFN-1 staining in (A) and (B) versus (G) and (H). Spreading of VAB-2/EFN-1-expressing cells was seen in 70% (135/192) of vab-1(e2) embryos. Mild spreading was seen in 33% (12/36) of control juIs53 embryos. | |||
|---|---|---|---|---|---|
| Name | F4.jpg | ||||
| Depict | Expr_pattern | Expr1106 | |||
| Expr1107 | |||||
| Anatomy | WBbt:0003679 | ||||
| WBbt:0003681 | |||||
| WBbt:0005829 | |||||
| WBbt:0006749 | |||||
| WBbt:0006750 | |||||
| WBbt:0006751 | |||||
| WBbt:0006759 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1999 | ||||
| Article_URL | DOI | id | 10.1016/S0092-8674(00)81675-X | ||
| Journal_URL | Cell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00003843 |
