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| WBPicture0000009525 | Description | Figure 4. PGL-1 Is a Constitutive Component of P Granules.Immunofluorescence micrographs of embryos, larvae, and extruded adult hermaphrodite gonads. Anterior is left; ventral is down.(A-D) Wild-type late 1-cell embryo costained with rabbit anti-PGL-1 antibody (A), mouse monoclonal antibody K76 (B), and the DNA dye, DAPI (D). The merged image (C) demonstrates double staining of granules by anti-PGL-1 and the original anti-P-granule monoclonal antibody, K76.(E-H) Wild-type early embryos stained with anti-PGL-1. P granules in the germline blastomere, P1 (E), P2 (F), P3 (G), and P4 (H) of a 2-, 4-, 8-, and 24-cell embryo, respectively, are stained.(I and J) Wild-type L1 (I) and L2 (J) stained with anti-PGL-1. P granules surround the germ nuclei.(K and L) Wild-type adult hermaphrodite gonad stained with anti-PGL-1 (K) and DAPI (L). P granules are perinuclear in the distal arm (upper left) and dissociate from the nuclear membrane during oogenesis (lower right).(M and N) pgl-1(bn101) adult hermaphrodite gonad costained with rabbit anti-PGL-1 (M) and mouse anti-GLH-1 (N). P granules fail to stain with anti-PGL-1 but stain with anti-GLH-1.(O-Q) glh-1 adult hermaphrodite gonad (O) and early embryos (P and Q) stained with anti-PGL-1. PGL-1 is present but not associated with P granules. Bars, 10 μm. | |||
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| Name | F4.jpg | ||||
| Depict | Expr_pattern | Expr1407 | |||
| Cellular_component | GO:0043186 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1998 | ||||
| Article_URL | DOI | id | 10.1016/S0092-8674(00)81605-0 | ||
| Journal_URL | Cell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00003186 |
