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WBPicture0000009516DescriptionFigure 3. MIX-1 Is Absent From Nondividing Somatic Cells in Wild-Type Males and Is Mislocalized to X in xol-1 Mutant Males. All panels are false-color confocal images. (A) A merged image of a wild-type adult male costained with propidium iodide (PI, red), and MIX-1 antibodies (green). MIX-1 is absent from all nondividing somatic cells but associates with all mitotic chromosomes and pachytene meiotic chromosomes within the one-armed gonad (green arrow).(B) A merged image of a wild-type adult hermaphrodite costained with PI (red) and MIX-1 (green) antibodies. The yellow subnuclear dots (white arrows) represent the MIX-1 X chromosome staining in somatic nuclei. MIX-1 also associates with all mitotic chromosomes and pachytene meiotic chromosomes of the two-armed gonad (green arrows). A role for MIX-1 in meiosis has not yet been defined.(C) A merged image of a him-5(e1490) dpy-21(e428); xol-1(y9) XO male costained with PI (red) and MIX-1 antibodies (green). The subnuclear, punctate MIX-1 staining pattern is similar to that of wild-type hermaphrodites, indicating that MIX-1 is inappropriately localized to X. In this one-armed gonad, mitotic and meiotic chromosome staining (green arrow) is normal. Scale bars, 100 μm.
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CropCrop_pictureWBPicture0000009517
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year1998
Article_URLDOIid10.1016/S0092-8674(00)80920-4
Journal_URLCell
Publisher_URLElsevier
ReferenceWBPaper00002999