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| WBPicture0000009495 | Description | Figure 4. LET-23-GFP Is Closely Associated with the Pn.p Cell Junctions. (A-C) Confocal left lateral images of a LET-23-GFP transgenic larvae in the L3 stage (1-3 hr after the L2 molt) showing LET-23-GFP expression in a subset of the Pn.p cells (P4.p, P5.p, P6.p, and P8.p) (A); cell junctions stained with the MH27 monoclonal antibody (B); or a merged image (C). LET-23-GFP is expressed as a thin lateral ring on the surface of Pn.p cells, which is slightly displaced toward the basal side of the cell junctions. LET-23-GFP is not expressed in P7.p, possibly owing to the expression of the 2nd cell fate.(D-F) Ventral view of an L3 larvae (at 3-4.5 hr after the L2 molt) showing LET-23-GFP expression in a lateral ring in P6.p (D), MH27 staining of cell junctions (E), and a merged image (F). Different focal planes showed that LET-23-GFP was not detectable on the apical or basal membrane domains of P6.p. The merged image (F) shows that LET-23-GFP (green) exhibits an annular distribution that is partly colocalized with (yellow), and partly basal to (green), MH27 staining. LET-23-GFP was expressed from the let-23 promoter in the gaIs27 transgenic array in a let-23(sy15) homozygous null animal. Scale bar is 10 um. | |||
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| Name | F4.jpg | ||||
| Depict | Expr_pattern | Expr688 | |||
| Anatomy | WBbt:0008117 | ||||
| WBbt:0008121 | |||||
| WBbt:0008125 | |||||
| WBbt:0008133 | |||||
| Cellular_component | GO:0005911 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1996 | ||||
| Article_URL | DOI | id | 10.1016/S0092-8674(00)81096-X | ||
| Journal_URL | Cell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00002436 |
