WormBase Tree Display for Picture: WBPicture0000009105

WBPicture0000009105 Description: Expression of pst-1 (A) Schematic of expression construct. The 20kb genomic environs of pst-1 (Wormbase, WB203) is shown. A yellow fluorescent protein (YFP) with a nuclear localization signal (NLS) under control of an intercistronic region directing SL2-splicing (red) is inserted after the stop codon for pst-1a in fosmid WRM063bF12. Black boxes denote coding exons of pst-1, gray boxes of the preceding genes within the operons (indicated as green bars), and white boxes denote coding exons of surrounding genes. Predicted start codons and direction of transcription are indicated by a rectangular arrow and stop codons by an asterisk. Straight arrows indicate the direction of transcription of partially shown genes. Approximate extent of the fosmid clones in kilobases (kb) is indicated on the left. SL2, splice leader 2; CEOP, C. elegans operon. (B) Expression of the pst-1 reporter at different developmental stages as shown. sc, seam cells; DIC, differential interference contrast; L1, first larval stage; phgc, pharyngeal gland cells. (D) Expression of pst-1 (left panel) and pst-2 (middle panel) appears mutually exclusive during the L2 larval stage (right panel). in, intestine; L2, second larval stage. Magnification is 400x in all panels except for the adult in B, which is 160x.
  Name: F5.large_A.jpg
  Crop: Cropped_from: WBPicture0000009104
  Depict: Expr_pattern: Expr8856
    Anatomy: WBbt:0005753
      WBbt:0005772
      WBbt:0005788
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Link to Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2009
    Article_URL: DOI id 10.1242/jcs.050732
    Journal_URL: JournalofCellScience
    Publisher_URL: TheCompanyofBiologists
  Reference: WBPaper00035477