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| WBPicture0000009027 | Description | Figure 2. DLG-1, a member of the MAGUK family of proteins, is localised at the subapical membrane of epithelial cells. (Ai) The genomic structure of the dlg-1 gene is shown underneath the scale bar, which corresponds to the coordinates in cosmid C25F6. White boxes correspond to the 12 coding exons and grey boxes at either end of the gene to the 5' and 3' untranslated regions (UTR), respectively. Note that the ATG is 291 nucleotides downstream of the previous ACeDB prediction, that exon 12 is 86 nucleotides longer at the 5'end than the previous ACeDB prediction, and that the stop site is 766 nucleotides upstream of the previous ACeDB prediction. Large black arrows show the localisation of primers used to generate a long-range PCR product that was tagged with a green fluorescent protein (GFP) cDNA. Arrowheads and small arrows show the localisation of T3-tagged primers used to generate dsRNA, against coding and 3'UTR sequence, respectively, for RNA interference (RNAi). (Aii) Schematic structure of the C. elegans DLG-1 protein and homologous proteins, Drosophila Lethal(1)Discs Large (accession no: P31007) and human presynaptic protein hDlg/SAP97 (accession no: NP_004078). Each protein possesses three PDZ domains (red boxes), 1 SH3 (green box) and 1 GUK (blue box) domain; percentages of amino acid identity/similarity between the individual domains of DLG-1 and its homologues are shown above each domain. The vertical black arrowhead indicates the position of GFP fusion. (B,C) Fluorescence and Nomarski views of uninjected and dlg-1(RNAi) dlg-1::gfp transgenic embryos demonstrating the strong reduction in DLG-1::GFP expression after injection with dsRNA against dlg-1. Both fluorescent images were taken using the same exposure time (1 second) after 8 hours of development. Confocal images showing an external (D) and internal (E) focal plane of a 1.5-fold dlg-1::gfp transgenic embryo after immunostaining with anti-GFP antibody. Weak background fluorescence could often be seen around the pharyngeal lumen and rectum (arrowheads in E; these expressing cells have not been identified). (F-H) GFP fluorescence of a young dlg-1::gfp transgenic adult showing expression in epithelial cells of the pharynx (arrow in F), intestine (arrowhead in F and large arrow in H), vulva (large arrow in G), uterus (arrowheads in G), spermatheca (long arrows in G), rectum (long arrow in H) and epidermis (arrowhead in H). Scale bars, 50 um (B,C,F-H); 10 um (D,E). | |
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| Name | F2.large.jpg | ||
| Crop | Crop_picture | WBPicture0000009028 | |
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Link to Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2001 | ||
| Journal_URL | JournalofCellScience | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00004765 |
