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| WBPicture0000008720 | Description | Figure 4. Immunolocalization of DPY-7 in the cuticle of larval and adult stages. (A-D) Nomarski (A and C) and fluorescence (B and D) images of two adults. A and B are in the some focal plane, as are C and D; C and D are at higher magnification. The alae are visible in A, running left to right across the specimen. The annuli are at right angles to the alae and extend to the edges of the animal. White arrows, a region of the cuticle where the regular spacing of the annuli is disrupted; every second delineating furrow is absent. The DPY-7 bands are seen to locate to the furrows. (E and F) Midstage larvae (L2 or L3), which do not have alae, stained with the DPY7-5a mAb (E) or double stained with the DPY7-5a and MH27 mAbs (F). White arrow, the boundary of an underlying seam cell detected with MH27. (G) DPY7-5a localization in an adult fixed by the modified Ruvkun method involving partial reduction of the specimen. The thread-like nature of the DPY-7 bands after partial reduction is evident. | |||
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| Name | F4.large.jpg | ||||
| Depict | Expr_pattern | Expr2553 | |||
| Anatomy | WBbt:0005733 | ||||
| Cellular_component | GO:0032154 | ||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1091/mbc.E02-08-0479 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00005815 |
