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| WBPicture0000008719 | Description | Figure 3. Immunolocalization of DPY-7 in C. elegans embryos. (A-F) DPY-7 localization in a comma stage embryo (A), a threefold embryo during cuticle secretion (B), and a late threefold embryo after cuticle secretion (C). (D-F) Nomarski images of the same embryos. In A, nuclei are seen as nonstained circles surrounded by a halo of DPY-7 fluorescence (white arrow). In B, some extracellular localized DPY-7 (red arrow) and some remaining perinuclear-localized DPY-7 (white arrow) are both seen. This animal seems to be in the process of secreting DPY-7. In C, secretion seems to be complete, with DPY-7 being detected in mature circumferential stripes. (G-I) Embryo double labeled with the DPY7-5a mAb (G and I) and anti-LIN-26 polyclonal antibody (H and I). LIN-26 is present in all hypodermal cell nuclei at this stage. White arrows, the three rows of cells that make up the main body hypodermis of one side of the animal are indicated: P cells that form the ventral hypodermis (bottom row); V cells that form the lateral seam (middle row); hyp-7 cells that form the main body hypodermal syncytium (top row). (J and K) Comma-stage embryo (J) and a late threefold embryo after cuticle secretion (K) double stained with DPY7-5a and MH27 mAbs. The hypodermal cell boundaries are visualized as thin lines by MH27 detection of adherens junctions. Arrow in J, the nucleus of one of the seam cells; the halo of DPY-7 localization is visible around it. By the late threefold stage, the hypodermal cells have elongated and the cuticle has polymerized on the hypodermal surface; arrows in K indicate the dorsal and ventral boundaries of an elongated seam cell. | |||
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| Name | F3.large.jpg | ||||
| Depict | Expr_pattern | Expr2553 | |||
| Anatomy | WBbt:0005733 | ||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1091/mbc.E02-08-0479 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00005815 |
