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WBPicture0000008708DescriptionFigure 3. Expression and localization of calcineurin. Images show only representative expression. CNA-1::GFP expressed in (A) ventral nerve cord (arrow) and vulva muscles (arrowhead) and (B) body-wall muscles of the midbody region. CNB-1::GFP expresses in spermatheca (C; arrowhead) and intestine (C; arrow). Nerve cord expression can also be observed in this image. (D) Immunostaining of wild-type worms with anti-CNB-1 antibodies detected CNB-1 in hypodermal seam cells (arrow). Bar, 20 um. (E-G) Immunogold electron micrograph (EM) showing the subcellular localization of CNB-1. (E) Transverse section through the midbody of a hermaphrodite showing CNB-1 localization as indicated by the signals of gold particles in the seam cells of the lateral hypodermis (LH) closely apposed to the cuticle (arrowhead). (F) Another region of seam cell showing signals. Lateral hypodermis (LH), cuticle (arrowhead), and excretory canal (arrow) are labeled. (G) Localization of CNB-1 in a wild-type male gonad. Distinct and scattered signals of CNB-1 are observed mainly in the cytoplasmic regions of the cellularized spermatids. Bar, 1 um.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2002
Article_URLDOIid10.1091/mbc.E02-01-0005
Journal_URLMolecularBiologyoftheCell
Publisher_URLTheAmericanSocietyForCellBiology
ReferenceWBPaper00005483