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| WBPicture0000008701 | Description | Figure 2. DLG-1 is localized to adherens junctions and required for AJM-1:: GFP localization. Immunostaining of embryos that express AJM-1:: GFP (green, A, D, G, J, M) with anti-PSD-95 antibodies (red, B, E, H) or with anti-PKC-3 antibodies (red, K, N). Nuclei are stained with DAPI (blue). Merged images (C, F, I, L, O) contain a 10 um scale bar. AJM-1:: GFP and DLG-1 colocalize to adherens junction on the epidermis (A-C, lima bean stage) and in the intestine (D-F, tadpole stage) of wild-type embryos. (G-I, twofold stage) AJM-1:: GFP is found in small punctate structures between cells in dlg-1(RNAi) embryos No DLG-1 protein is detected in these embryos. (J-L, gastrulating embryo) AJM-1:: GFP is found at intestinal adherens junctions (green) whereas PKC-3 is localized to the apical surface of these cells (red) in wild-type embryos. (M-O, gastrulating embryo) AJM-1:: GFP is found in punctate structures, but PKC-3 is found at the apical surface of cells from dlg-1(RNAi) embryos. | |||
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| Name | F2.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000008702 | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2001 | ||||
| Article_URL | DOI | id | 10.1091/mbc.12.11.3465 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00004983 |
