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WBPicture0000008673DescriptionFigure 4. (A-C) In vivo ric-19 promoter expression visualized with the use of the NLS-GFP or NLS-lacZ reporter genes. (A) NLS-lacZ staining in an animal transgenic for the array Ex[pCelicaDP-lacZ; pRF4]. (B) NLS-GFP expression in a young adult transgenic animal carrying the array Ex[pCelicaDP-GFP; pRF4]. (C) Nomarski view of panel b. hg, head ganglia; vnc, ventral nerve cord; tg, tail ganglia. Bars in A-C, 50 um. (D-I) Confocal microscopy of transgenic animals carrying the array Ex[pRIC19::GFP; pRF4]. (D-F) Three confocal planes of the head region. In D, the excretory canal cell and gland cells are indicated; the autofluorescent gut granules (gg) are also indicated. Note the expression of the RIC-19::GFP protein in neurons of the head ganglion (most visible in E) as well as the high levels of expression in a pharyngeal interneuron (E) and m2 neurons (F). (G) A portion of the ventral nerve cord; note that the RIC-19::GFP fusion protein is expressed in the cytoplasm of each neuron shown. (H and I) Two sections of the tail region; note the expression of the RIC-19::GFP protein in the neurons of the tail ganglia. Bars in D-I, 20 um.
NameF4.large.jpg
DepictExpr_patternExpr1071
Expr1072
AnatomyWBbt:0003634
WBbt:0003668
WBbt:0005135
WBbt:0005775
WBbt:0005776
WBbt:0005829
WBbt:0006977
Cellular_componentGO:0005737
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2000
Article_URLDOIid10.1091/mbc.11.10.3277
Journal_URLMolecularBiologyoftheCell
Publisher_URLTheAmericanSocietyForCellBiology
ReferenceWBPaper00004371