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WBPicture0000008648 Description: Figure 7. CeKinesin-II and CeOsm-3 polypeptides immunolocalize to and concentrate in the ciliated endings of chemosensory neurons. Growing cultures of mixed stage N2 wild-type worms were fixed and permeabilized, then used to immunolocalize the CeKRP95, Osm-3, and CeKAP polypeptides. Shown are confocal projections of L2-L4 stage worms stained with anti-CeKRP95 antibodies (A-C), anti-Osm-3 antibodies (D-F), and anti-CeKAP antibodies (G-I). CB, cell body; Ci, sensory cilia. (A-F) Perinuclear, punctate staining of the polypeptides is observed in the cell bodies and along dendrites of the amphid chemosensory neurons. This pattern is seen in immunolocalization experiments using all three antibodies but is most obvious in anti-Osm-3- and anti-CeKRP95-stained worms. All antibodies demonstrate intense staining in the ciliated endings of the chemosensory neurons (A-I). Although all three antibodies stain the cell bodies and dendrites of these neurons in a punctate manner, the most intense staining is seen in the ciliated endings, suggesting a concentration of the CeKinesin-II and CeOsm-3 holoenzymes in these structures.
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  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1999
    Article_URL: DOI id 10.1091/mbc.10.2.345
    Journal_URL: MolecularBiologyoftheCell
    Publisher_URL: TheAmericanSocietyForCellBiology
  Reference: WBPaper00003466