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| WBPicture0000008642 | Description | Figure 5. ZEN-4 localization is cell cycle dependent. Embryos are coimmunostained with ZEN-4N to label ZEN-4 (red) and N357 (green) to label the microtubules. Anterior is to the left in all panels. (A) ZEN-4 staining in a two-cell embryo. (B) Tubulin staining in the same embryo shown in panel A. (C) Combined images of panels A and B, showing the overlapping pattern in yellow. ZEN-4N strongly stains the midbody remnant from the first cell division (white arrow), and expression can also be seen at the periphery of the chromosomes in the more posterior cell and in the central region of the midzone in the anterior anaphase cell (white arrowhead). The chromatin can be seen as the black region of the spindle, which excludes both antibodies (white caret). (D) ZEN-4 staining in the midbody of a dividing cell. (E) Tubulin staining of the same embryo shown in panel D. (F) Overlay of panels D and E, showing that ZEN-4 colocalizes with the midzone microtubules at the cytoplasmic bridge between daughter cells. | |||
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| Name | F5.large.jpg | ||||
| Depict | Expr_pattern | Expr1412 | |||
| Cellular_component | GO:0005813 | ||||
| GO:0051233 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 1998 | ||||
| Article_URL | DOI | id | 10.1091/mbc.9.8.2037 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00003163 |
