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| WBPicture0000008641 | Description | Figure 4. ZEN-4 is required for nor- mal organization of the midzone microtubules. Embryos are coimmunos- tained with ZEN-4N to label ZEN-4 (red), N357 to label tubulin (green), and DAPI (blue) to label DNA. (A) ZEN-4 staining during the first cell division. ZEN-4 localizes in discrete short lines in the spindle midzone. (B) Tubulin and DNA staining in the same embryo shown in panel A. A white arrowhead points to organized microtubules in the spindle midzone. (C) An overlay of panels A and B, showing that ZEN-4 colocalizes with the microtubules between the separating chromosomes. (D) ZEN-4N does not stain the offspring of a germ line mosaic mutant, indicating that the w35 allele encodes a protein null. (E) Tubulin and DNA staining of the same embryo shown in panel D. A white arrowhead points to the midzone, which has greatly reduced numbers of microtubules. (F) ZEN-4N recognizes a major band of 93 kDa in extracts of total worm proteins. This is slightly greater than the predicted size of 87 kDa for ZEN-4A and ZEN-4B. | |||
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| Name | F4.large.jpg | ||||
| Depict | Expr_pattern | Expr1412 | |||
| Cellular_component | GO:0005813 | ||||
| GO:0051233 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 1998 | ||||
| Article_URL | DOI | id | 10.1091/mbc.9.8.2037 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00003163 |
