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| WBPicture0000008633 | Description | Figure 2. Distribution of CeE/DA during embryogenesis. Ubiquitous, nuclear-localized CeE/DA is observed in early embryogenesis. Equal levels of CeE/DA are detected in all metaphase nuclei; a diminution in the signal is observed in nuclei entering and exiting mitosis. Different levels of background cytoplasmic staining are observed between embryos of both similar and different developmental stages and appear to be related to fixation variability rather than to CeE/DA protein concentration. (A) A 2-cell embryo; (B) a 4-cell embryo; (C) an approx. 24-cell embryo; (D) an approx. 100-cell embryo. CeE/DA becomes progressively restricted to mostly neurons and their precursors beginning at mid-embryogenesis, as shown in (E-H). (E) Dorsal (top) and ventral (bottom) view of an embryo at approx. 260 minutes (approx. 300 cells) after the first cleavage. (F) Dorsal (top) and ventral (bottom) view of a bean-stage embryo. (G) Lateral view of a 1.5-fold embryo stained with CeE/DA antibody (top) and DAPI (bottom), showing that CeE/DA is confined primarily to neurons in the head, ventral nerve cord and tail. (H) Pretzel-stage embryo stained with CeE/DA antibody (top) and DAPI (bottom), showing restriction of CeE/DA to only a few cells in the head and tail of the animal. There are 14 positive nuclei in the head and seven positive nuclei in the tail, three of which are seen in this focal plane (arrows). The mostly neuronal distribution of CeE/DA during mid-embryogenesis is emphasized in (I-K). (I) Reproduction of the dorsal (top) and ventral (bottom) views of nuclei in an embryo 260 and 270 minutes after the first cleavage, respectively (Sulston et al., 1983). Neurons and their immediate precursors have been colored orange; three dorsal neuronal CeE/DA-positive nuclei are coloured green for comparison with the antibody-staining pattern. (J) Dorsal (top) and ventral (bottom) view of an embryo at about 260 minutes of development stained with the CeE/DA antibody. White arrows mark the three nuclei corresponding to the triplet coloured green in I. (K) DAPI stain of focal planes shown in B. Yellow arrows mark the ridge of hypodermal nuclei present on the dorsal side of embryos at this stage. Scale bar, 10 µm. | |
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| Name | FigB.jpg | ||
| Remark | Ubiquitous, nuclear-localized CeE/DA is observed in early embryogenesis. | ||
| Depict | Expr_pattern | Expr1470 | |
| Anatomy | WBbt:0004017 | ||
| Cellular_component | GO:0005634 | ||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1997 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00002826 |
