WormBase Tree Display for Picture: WBPicture0000008629

WBPicture0000008629 Description: Figure 2. Dynamic pattern of HLH-2 protein accumulation during the AC/VU decision. (A-F) Confocal micrographs of L2 hermaphrodite gonads. Hermaphrodites stained with antibodies against HLH-2 (green) and LacZ (red); cells in which both antibodies are detected appear yellow. Small yellow arrows indicate Z1.ppp and Z4.aaa. Larger white arrows indicate HLH-2 expression in the presumptive AC. Distal tip cells are marked with an asterisk. Photomicrographs are arranged in an inferred temporal sequence, on the basis of the observation that lin-12::lacZ and lag-2::lacZ are initially expressed in both Z1.ppp and Z4.aaa, but become restricted to the presumptive AC (lag-2::lacZ expressing) or presumptive VU (lin-12::lacZ expressing). Only (and all) animals that showed HLH-2 expression in both distal tip cells as well as lin-12::lacZ expression in all six vulval precursor cells (Wilkinson and Greenwald 1995) or lag-2::lacZ expression in both distal tip cells (Henderson et al. 1994) were counted, to be certain that the staining procedure had worked well in each animal scored. (A,C,E) lin-12::lacZ animals (full genotype: smg-1(r861) unc-54(r293); arIs11[lin-12::lacZ]). A total of 36 animals were observed with lin-12::lacZ in both Z1.ppp and Z4.aaa. Of these, 20/36 animals showed no HLH-2 accumulation in either cell (A), and 16/36 showed HLH-2 accumulation in one cell (C). Seven animals were observed in which lin-12::lacZ expression was already restricted to the presumptive VU. Of these, 7/7 showed HLH-2 accumulation in the other cell, the presumptive AC (E). (B,D,F) lag-2::lacZ animals (full genotype: smg-1(r861) unc-54(r293); arIs13[lag-2::lacZ]). Seven animals were observed with lag-2::lacZ expressed in both Z1.ppp and Z4.aaa. Of these, 2/7 animals showed no HLH-2 accumulation in either cell (B), and 5/7 showed HLH-2 accumulation in one cell (D). A total of 21 animals were observed in which lag-2::lacZ expression was restricted to the presumptive AC. Of these, 21/21 showed HLH-2 accumulation costaining the presumptive AC (F).
  Name: F2.large.jpg
  Depict: Expr_pattern: Expr2824
    Anatomy: WBbt:0007628
      WBbt:0007667
      WBbt:0007688
      WBbt:0007707
  Acknowledgment: Template: WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2003
    Article_URL: DOI id 10.1101/gad.1160803
    Journal_URL: Genes&Development
    Publisher_URL: ColdSpringHarborLabPress
  Reference: WBPaper00006303