WormBase Tree Display for Picture: WBPicture0000008488
expand all nodes | collapse all nodes | view schema
| WBPicture0000008488 | Description | Figure 3. GPR-1/GPR-2 localize to the mitotic spindle and cell cortex in a LIN-5-dependent manner. DNA staining with DAPI (blue) and immunostaining of GPR-1/GPR-2 (green) and LIN-5 (red). Merged images are shown in D, H, L, P,T, and X. (A-D) Fertilized embryo in meiosis II. LIN-5, but not GPR-1/GPR-2, showed specific localization to the meiotic spindle (arrowheads, B,C). (E-H) One-cell embryo in metaphase. GPR-1/GPR-2 (F) and LIN-5 (G) colocalize to the spindle asters (arrowheads) and kinetochore MTs (arrows). (I-L) Two-cell embryo. GPR-1/GPR-2 (J) and LIN-5 (K) colocalize to the spindle apparatus as well as the cell cortex (arrowheads). (M-P) Four-cell embryo. In addition to spindle staining, both proteins showed stronger staining at the P2/EMS boundary (arrowhead) than at other cell membranes (arrow). (Q-X) Localization in RNAi embryos. (Q-T) GPR-1/GPR-2 staining is strongly reduced but not fully eliminated in gpr-1/gpr-2(RNAi) embryos. (S) LIN-5 staining remained at the spindle apparatus but appeared reduced at the cell periphery. (U-X)lin-5 RNAi eliminated LIN-5 staining (W) and disrupted GPR-1/GPR-2 localization (V). Bar, 10 μm. | |||
|---|---|---|---|---|---|
| Name | F3.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000008489 | |||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1101/gad.1081203 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00005878 |
