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| WBPicture0000008474 | Description | Figure 6. LIN-11 protein expression and localization are regulated by SUMO modification. (A-F) Expression of the lin-11::GFP reporter in the pi cells of wild-type and smo-1(ok359) animals. Nomarski photomicrographs (A,C,E) and corresponding fluorescence images (B,D,F), lateral views. (A,B) A wild-type mid-L4 stage vulva. GFP expression is shown in the pi cells (asterisk) and secondary-fate vulval cells (arrow). The six pi cells shown are arranged in two lines (outline). (C,D) A smo-1(ok359) animal at mid-L4 stage. (C) The unfused anchor cell is indicated by an arrowhead. (D) GFP expression is weaker and nuclear labeling decreased relative to the cytoplasmic labeling. The pi cells are clustered (outline). (E,F) A smo-1(ok359) animal at late L4 stage. Weak expression is detected only in one uterine pi cell (outline). (G) Immunofluorescence staining with MH27 and the anti-myc antibodies of a transgenic smo-1(ok359) animal expressing the plin-11::MYC::SUMO::LIN-11 fusion protein. The upper and lower panels show the two sides of the uterus. Note six pi cells in each side of the uterus (star). LIN-11 is expressed in discrete nuclear dots (arrowhead). Arrow indicates one of the vulval cells. Bar, 10 μm. | |||
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| Name | F6.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000008475 | |||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2004 | ||||
| Article_URL | DOI | id | 10.1101/gad.1227104 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00024467 |
