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| WBPicture0000008451 | Description | Figure 5. Expression and stress-induced nuclear accumulation of SKN-1::GFP. (A) SKN-1::GFP transgenes. (a) skn-1 gene. Transcribed coding and untranslated regions are indicated in red and blue, respectively. (b) SKN-1::GFP translational fusion construct, which includes an EcoRI fragment that previously rescued maternal skn-1 lethality (Bowerman et al. 1992). C. elegans DNA is indicated by a black line. (c) SknPro::GFP promoter fusion, in which the 38 N-terminal SKN-1 amino acids are fused to GFP containing a nuclear localization signal. (B-D) Embryonic expression of SKN-1::GFP. Nomarski (left) and fluorescent (right) views of 100-cell (B), 280-min (C), and threefold (D) embryos. Endogenous intestinal autofluorescence is visible as yellow or orange (see Materials and Methods). White triangles, intestine precursor nuclei; int, intestine; ph, pharynx. (E) SKN-1::GFP expression in ASI neurons (arrows). Nomarski/fluorescent (left) and fluorescent (right) views are shown of a typical DiI-exposed L4 larva. (F) Larval SKN-1::GFP expression under normal conditions. (Bottom) Fluorescent and Nomarski closeups of the boxed central region of this L2. An intestinal nucleus is indicated by a white triangle, and the ASI neurons (arrows) are shown in detail in the top right box. (G) SKN-1::GFP localization under oxidative stress. A heat-shocked L2 is shown, but similar results were obtained after exposure to other oxidative stress inducers (Table 3). The integrated strain Is007 is shown, but two extrachromosomal lines and a different integrated line exhibited similar patterns. | |||
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| Name | F5.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000008452 | |||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1101/gad.1107803 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00006024 |
