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| WBPicture0000008449 | Description | Figure 2. skn-1-dependent GCS-1::GFP expression in the intestine and ASI neurons. (A-F) GCS-1::GFP expression in wild-type animals. A gcs-1 genomic fragment containing its 17 N-terminal codons and 1840 upstream base pairs was fused to the N terminus of GFP, which contained a nuclear localization signal. The expression patterns shown are each representative of more than two independent transgenic lines, and of all postembryonic stages examined (L2-adult; data not shown). (A,B) Nomarski (A) and fluorescent (B) views of an L2 larva. (B) A line demarcates the approximate boundary between the anterior intestine (I) and posterior pharynx (P). (C,D) Combined Nomarski/fluorescent (C) and fluorescent (D) views of the head of a typical L4-stage animal that had been exposed to DiI. (D) One of the two ASI neurons is indicated with an arrow. (E,F) An L2 larva in which GCS-1::GFP expression was induced to high levels in the intestine by heat. A similar induction occurred in response to paraquat (Table 2). The boundary between the anterior intestine and posterior pharynx is indicated as in B. (G-L) GCS-1::GFP was not detectable outside of the pharynx in skn-1 homozygotes. Typical animals are shown from experiments that parallel those displayed to the left in A-F. Note the absence of GCS-1::GFP in the intestine and ASI neurons under normal conditions (G-J), and after treatment with heat (K,L) or paraquat (data not shown). In two independent transgenic lines, in a homozygous skn-1 background GCS-1::GFP expression was not detected in these tissues in any animals under either normal or induction conditions. | |||
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| Name | F2.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000008450 | |||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1101/gad.1107803 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00006024 |
