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| WBPicture0000008431 | Description | Figure 6. Condensin components colocalize with centromere components and are required for the restricted distribution of centromere proteins toward the spindle poles during mitosis. (A-F) Wild-type prometaphase (A-C) and metaphase (D-F) chromosomes stained with DNA dye (blue), and antibodies against SMC-4 (green) and centromere protein HCP-3 (red). Merged images (C,F) show that SMC-4 and HCP-3 colocalize. The magnified focus of staining from the metaphase plate shows overlap of the two proteins (F, inset). (G-I) Metaphase chromosome stained with DNA dye (blue), and antibodies to SMC-4 (green) and the kinetochore centrosome-associated protein CeMCAK (red). In the merged image and magnified inset (I), CeMCAK partially overlaps (yellow) SMC-4 and extends more poleward (red) than SMC-4 on metaphase chromosomes. Unlike SMC-4, CeMCAK stains the centrosomes (H, arrowheads). (J) Wild-type and (K)smc-4(RNAi) two-cell embryos stained with tubulin antibody (blue), DNA dye (red), and HCP-3 antibody (green; yellow represents overlap between HCP-3 and DNA). HCP-3 associates with mitotic chromosomes when SMC-4 is depleted, but is not present in a restricted pattern facing the spindle poles. (L) Wild-type and (M) hcp-3(RNAi) one-cell embryos stained with tubulin antibody (blue), DNA dye (red), and SMC-4 antibody (green; yellow represents overlap between SMC-4 and DNA). When HCP-3 is depleted, SMC-4 associates with DNA but in a disorganized pattern. | |||
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| Name | F6.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000008432 | |||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2002 | ||||
| Article_URL | DOI | id | 10.1101/gad.968302 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00005173 |
