WormBase Tree Display for Picture: WBPicture0000008396

WBPicture0000008396 Description: Figure 4. Immunolocation of MEI-2. (A) MEI-2 expression was first apparent in the proximal gonad of adult hermaphrodites. Typically, the last 2-3 oocytes in this region stained with anti-MEI-2. MEI-2 was not observed in the distal portion of the gonad (*), where mitotic proliferation of germ cells occurs. A dotted line indicates the outline of one gonad arm (as determined by Nomarski microscopy and DAPI immunofluoresence, not shown). (B) An embryo undergoing meiosis II is shown. MEI-2 antiserum decorated the meiotic spindle and condensed meiotic chromatin (clear arrowhead), the polar body from meiosis I (arrow) and the condensed sperm pronucleus (white arrowhead). Anti-MEI-2 also stained the meiosis-I spindle and polar body from meiosis II (not shown). With the exception of the polar bodies, all MEI-2 staining disappeared after the completion of meiosis (see Fig 6C). (C-E) Deconvolution imaging of a meiotic spindle in metaphase is shown, stained with (C) DAPI, to visualize chromatin, (D) anti-alpha-tubulin, and (E) anti-MEI-2. The MEI-2 staining patterns were also documented in the absence of DAPI and alpha-tubulin staining (data not shown), to ensure that the observed patterns did not result from artifacts of multichannel fluorescence microscopy. Bars: A and B10 μm; E 1 μm.
  Name: F4.large.jpg
  Depict: Expr_pattern: Expr974
    Anatomy: WBbt:0005175
    Cellular_component: GO:0000794
      GO:0000922
  Acknowledgment: Template: WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2000
    Journal_URL: Genes&Development
    Publisher_URL: ColdSpringHarborLabPress
  Reference: WBPaper00004129