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| WBPicture0000008395 | Description | Figure 3. Expression of mei-2 mRNA and protein. (A) Northern blot analysis of mei-2 mRNA. Poly A+ RNA was loaded into each lane and probed with a genomic clone containing mei-2 (see Materials and Methods). RNA was harvested from gravid wild-type hermaphrodites, larval stages L1, L2/L3, L4, as well as glp-1 hermaphrodites (lacking a germ line), fem-3 adults (which produce sperm, but not oocytes), and fem-1 adults (which do not produce sperm, and tend to bloat with unfertilized oocytes). As a loading control, the blot was probed simultaneously with rp21, a ribosomal protein gene. fem-1(lf) lane exposure was adjusted until therp21 signal was approximately equal to the signal in other lanes. (B) Western blot analysis of MEI-2. Homogenates prepared from mei-2 mutant hermaphrodites, wild type,fem-3, and fem-1 were probed with anti-MEI-2 serum. Note the slightly retarded migration of MEI-2(sb31) protein, consistent with the net insertion of four amino acids. As a loading control, the blot was probed simultaneously with anti-alpha-tubulin. | |
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| Name | F3.large.jpg | ||
| Depict | Expr_pattern | Expr974 | |
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2000 | ||
| Journal_URL | Genes&Development | ||
| Publisher_URL | ColdSpringHarborLabPress | ||
| Reference | WBPaper00004129 |
