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WBPicture0000008246DescriptionC, RT-PCR of developmental stage-specific RNA amplified with fre-1-specific primers. ahh was used as an internal control (see Experimental Procedures). E, embryo; A, adult; M22, mixed stage grown at 22 C; M33, mixed stage heat-treated at 33 C for 2 h; D, dauer; L, DNA ladder; (+), positive control containing fre-1 and dcs-1 cDNAs; (-), no template. Note that fre-1 is detected in all stages except the embryo. When mixed stages were compared before and after heat treatment, both genes showed a higher expression following the heat treatment.
NameF4.large_B.jpg
CropCropped_fromWBPicture0000008244
DepictExpr_patternExpr2644
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2003
Article_URLDOIid10.1074/jbc.M306355200
Journal_URLTheJournalofBiologicalChemistry
Publisher_URLTheAmericanSocietyForBiochemistryandMolecularBiology
ReferenceWBPaper00006128