WormBase Tree Display for Picture: WBPicture0000008215

WBPicture0000008215 Description: Figure 6. The relative RT-PCR of CE2FT-1 mRNA from C. elegans at different stages. A, total C. elegans RNA from the populations of L1, L2-L4, and adult stage were prepared and used as templates in first strand cDNA synthesis. A 500-bp positive control from the RT-PCR kit was used as a control for the RT-PCR method (Line Positive Control). The arrows indicate the expected size of alpha-actin and CE2FT-1. Size markers in bp are indicated. B, the quantitative real-time RT-PCR of CE2FT-1 mRNA from C. elegans at different stages. Total C. elegans RNA from the populations of L1, L2-L4, and adult stage were prepared and used as templates in first strand cDNA synthesis. A 106-bp cDNA was amplified. The expression level was indicated by threshold cycles. C, confirmation of a single product of amplification in quantitative real time RT-PCR was performed on 1.2% agarose gel. A 106-bp cDNA fragment was amplified from CE2FT-1, and a 100-bp cDNA fragment was amplified from actin. Size markers in bp are indicated.
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  Depict: Expr_pattern: Expr2254
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2002
    Article_URL: DOI id 10.1074/jbc.M207487200
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00005523