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WBPicture0000008211DescriptionFigure 4. Immunofluorescent localization of PHY-1, PHY-2, PDI-2, and DPY-7 in C. elegans embryos. 1.5-Fold (A) and elongated (B) wild-type embryos were incubated with rabbit polyclonal antibodies against the PHY-1, PHY-2, or PDI-2 polypeptides, and with a mouse monoclonal antibody against DPY-7 collagen polypeptide, and stained with AF594-labeled anti-rabbit IgG (red) and AF488-labeled anti-mouse IgG (green). A, in the 1.5-fold embryos all four polypeptides were colocalized in the hypodermal cells. B, in the elongated embryos the hypodermal location is maintained for the P4H subunits PHY-1, PHY-2 and PDI-2, but DPY-7 was incorporated into the developing cuticle.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2002
Article_URLDOIid10.1074/jbc.M203824200
Journal_URLTheJournalofBiologicalChemistry
Publisher_URLTheAmericanSocietyForBiochemistryandMolecularBiology
ReferenceWBPaper00005422