WormBase Tree Display for Picture: WBPicture0000008168

WBPicture0000008168 Description: Figure 6. Immunostaining of VHA-6. Embryos at different cleavage stages (A, 4 cells; C, 4 and 32 cells; and D, 200 cells), ones at the comma stage (E), 2-fold embryos (F), L1 larvae (G), and adult worms (H and I) were fixed and then incubated with the affinity-purified anti-VHA-6 antibodies. A-C, in early embryos, VHA-6 was clearly detectable on dot-like structures (arrows) in a germ line cell lineage. B is a phase-contrast image of A: ABa, ABp, EMS, and P2 cells were identified by their morphology.D, after the cleavage stage (200 cells), VHA-6 was expressed in two kinds of cells (arrows). E, at the comma stage, the two types of cells highly expressing VHA-6 (arrows) were aligned on both lateral sides. F,in 2-fold embryos, VHA-6 was strongly observed on the luminal sides (arrowheads) of the intestine. G, in L1 larvae, VHA-6 was strongly expressed on the apical surface of intestinal cells (arrowhead). H and I, in addition to intestinal cells, VHA-6 was also detectable at the junction between the pharynx and the intestine (arrows) of adult worms. I is a higher magnification of H. Dashed lines indicate the edges of the worm body. The area denoted by an asterisk represents the intestinal lumen of the worm.Scale bars, 20 μm (A, C, D, E, andF) or 50 μm (G-I).
  Name: F6.large.jpg
  Depict: Expr_pattern: Expr2407
    Anatomy: WBbt:0005767
      WBbt:0005772
      WBbt:0006873
    Cellular_component: GO:0045177
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2001
    Article_URL: DOI id 10.1074/jbc.M101652200
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00004842