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WBPicture0000008157 Description: FIG. 5. C kinase adapter protein accumulation and CKA1 gene promoter activity in situ. A, the location of CKA1/CKA1S in embryos was determined by immunofluorescence analysis. Fluorescence signals that correspond to CKA1/CKA1S-IgG complexes are enriched along segments of the cell periphery that are involved in cell-cell junctions (arrows). A 4-cell embryo is shown. B, locations of nuclei in the embryo shown in A are revealed by staining with diamidophenylindole, which intercalates into DNA. Chromosomes in two nuclei are associated with the mitotic spindle apparatus. Positions of plasma membranes in the 4-cell embryo (A) were determined by interference microscopy and traced to generate the diagram in C. Comparison of A and C reveals that CKA1/CKA1S accumulate at junctions of cell 3 with cells 4, 1, and 2, and at points of contact between cells 1 and 2. A representative 4-cell embryo is shown in A-C. Other examples of this pattern of adapter protein accumulation were observed in independent immunostaining experiments. D, transgenic C. elegans that carry the GFP reporter gene under the regulation of CKA1 promoter/enhancer were fixed with paraformaldehyde. Fluorescence signals emanating from GFP in the nucleus were observed and recorded with a Nikon Eclipse 400 microscope. Robust CKA1 promoter activity was evident in both the large nuclei of (>10) intestinal (gut) cells and smaller nuclei located in anal depressor and sphincter muscle cells and cells of the intestinal-rectal valve. A modest level of CKA1 gene transcription was detected in pharyngeal muscle nuclei. (The intensity of fluorescence signals near the pharynx (bounded by the white rectangle) was enhanced 2.5-fold so that high level accumulation of GFP in gut nuclei and the lower abundance of GFP in nuclei of pharyngeal cells could be visualized in a single photomicrograph.) A representative L3 nematode is shown. Similar patterns of CKA1 gene transcription were obtained for both adults and larvae in several experiments.
  Name: F5.large.jpg
  Depict: Expr_pattern: Expr845
      Expr846
    Anatomy: WBbt:0004292
      WBbt:0005451
      WBbt:0005772
      WBbt:0005797
      WBbt:0005798
    Cellular_component: GO:0005938
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2001
    Article_URL: DOI id 10.1074/jbc.M008990200
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00004638