WormBase Tree Display for Picture: WBPicture0000008146

WBPicture0000008146 Description: Figure 7. Developmental expression of asp-1 mRNA. In A and B, C. elegans were fixed, permeabilized, and then hybridized with digoxigenin-labeled, ASP-1 antisense DNA as described under Experimental Procedures. RNA-DNA complexes were visualized by incubating the specimens serially with antidigoxigenin IgGs coupled to alkaline phosphatase and a chromogenic substrate. Alkaline phosphatase catalyzes the synthesis of an insoluble reaction product (shown as a black precipitate) in cells expressing ASP-1 mRNA. A, an L1 larva with ASP-1 mRNA located exclusively in the intestine. B, an L2 larva expressing ASP-1 mRNA in the intestine. The level of ASP-1 expression is significantly lower than that observed in younger nematodes. Arrows labeled ph and G indicate the posterior end of the pharynx and the developing gonad, respectively. C, total RNA was prepared from C. elegans embryos, L1 larvae, and adults and then subjected to Northern blot analysis. The amount of 32P-labeled ASP-1 probe bound was quantified by PhosphorImager analysis and then normalized to the level of myosin light chain mRNA as described previously (33). The data are means and standard deviations from three independent experiments.
  Name: F7.large.jpg
  Depict: Expr_pattern: Expr1090
      Expr1091
    Anatomy: WBbt:0005772
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2000
    Article_URL: DOI id 10.1074/jbc.M000956200
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00004299