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| WBPicture0000008143 | Description | Figure 6. Immunolocalization of HSP25 in C. elegans. Nematodes were stained using rabbit polyclonal antibody to HSP25 in combination with mouse monoclonal antibodies to nematode β-integrin (MH25) or to body wall muscle M-lines (MH42) (37), followed by fluorescein isothiocyanate-conjugated anti-rabbit secondary antibody and/or Texas Red-conjugated anti-mouse secondary antibody. A, anterior of an adult, stained with antibody to HSP25. Note the striated pattern seen in body wall muscle.B, localization of HSP25 to dense bodies (db) and M-lines (m); the dark line corresponds to a cell boundary (cb) with an adjacent muscle cell. C, anti-β-integrin also stains dense bodies and M-lines, as well as cell boundaries. D-F, co-localization of HSP25 and β-integrin. The staining patterns of HSP25 (D) and β-integrin (E) coincide in both the dense bodies and M-lines as shown in the superimposed image, F. G-I, co-localization of HSP25 and MH42. The staining patterns of HSP25 (G) and MH42 (H) coincide in M-lines (I). J and K, pharyngeal staining pattern of HSP25 in L2 and adult, respectively. L, staining of HSP25 at junctions between cells of the spermathecal wall.M, the animal was stained with anti-HSP25 in the presence of excess recombinant HSP25 as a competitor, together with DAPI (diamidinophenylindole) to reveal cell nuclei; note the absence of detectable HSP25 signal. N, the same animal as in panel M, with staining pattern of M-lines to ensure the animals had been permeabilized. O and P, same conditions as in M and N, respectively, at a lower magnification, showing a view of the anterior of the animal. | |||
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| Name | F6.large.jpg | ||||
| Depict | Expr_pattern | Expr1088 | |||
| Anatomy | WBbt:0005813 | ||||
| Cellular_component | GO:0031430 | ||||
| GO:0055120 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2000 | ||||
| Article_URL | DOI | id | 10.1074/jbc.275.13.9510 | ||
| Journal_URL | TheJournalofBiologicalChemistry | ||||
| Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
| Reference | WBPaper00003970 |
