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WBPicture0000008130DescriptionNorthern blot analyses of GnT I mRNA expression during C. elegans development. Total RNA (~20 μg/lane) and poly(A)+ RNA (~2.5 μg/lane) were probed with gly-12 cDNA. The blots were stripped with boiling 0.1% SDS and reprobed with fem-1 cDNA as a sample loading control. RNA was obtained from embryos (E), the four larval stages (L1-L4), adult worms (Ad), and a mixture of all stages (Mix). The ~2.1-kb gly-12 mRNA was expressed in all developmental stages, with the highest level in embryos and the lowest in adults.
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CropCropped_fromWBPicture0000008129
DepictExpr_patternExpr651
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1999
Article_URLDOIid10.1074/jbc.274.1.288
Journal_URLTheJournalofBiologicalChemistry
Publisher_URLTheAmericanSocietyForBiochemistryandMolecularBiology
ReferenceWBPaper00003352