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WBPicture0000008128 | Description | Figure 6. Expression of vha-3 in gastric and intestinal cells. Transgenic worms carrying different expression plasmids were permeabilized and stained with 5-bromo-4-chloro-3-indolyl-β-D-galactoside (28). A, a transgenic worm harboring pCV3-03, the lacZ reporter gene under control of the 2.2-kb upstream regulatory region of vha-3. Staining is seen in the H-shaped excretory cell, intestine, and hypodermis. B, a shorter staining of the transgenic worm carrying pCV3-03. The staining of cells in the head and tail were clearly detectable. C, an adult worm carrying pCV02, the lacZ gene under control of the upstream regulatory region of vha-1 and vha-2. The lacZ fusion gene was expressed in the H-shaped excretory cell and rectum as reported previously with GFP constructs (18). Dark-stained spots correspond to cell nuclei. Nuclei and canals of the H-shaped excretory cell are indicated by arrows and arrowheads, respectively. | |||
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Name | F6.large.jpg | ||||
Depict | Expr_pattern | Expr1401 | |||
Anatomy | WBbt:0005733 | ||||
WBbt:0005792 | |||||
WBbt:0005812 | |||||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 1998 | ||||
Article_URL | DOI | id | 10.1074/jbc.273.35.22570 | ||
Journal_URL | TheJournalofBiologicalChemistry | ||||
Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
Reference | WBPaper00003195 |