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WBPicture0000008100 Description: Figure 5. Effects of stress and stage of development on expression of SEC-1 mRNA. Northern blots that contain size-fractionated RNAs from C. elegans were prepared as described under Experimental Procedures. Blots were probed with 32P-labeled SEC-1 cDNA corresponding to nucleotides 58-319 in Fig. 1. Hybridization of the probe with (0.65 kb) SEC-1 mRNA was visualized by autoradiography. A, fractionated RNAs (4 μg) from control C. elegans (lane 2) and C. elegans incubated with 0.2 mM CdCl2 for 24 h (lane 1) were analyzed. B, lanes of a Northern gel received 4 μg of total RNA from either embryos (E) or larvae (L1-L4) at various stages of postembryonic development. C (upper part), Northern blot analysis was performed on samples of total RNA (20 μg) isolated from control C. elegans maintained at 20 C (lane 3) or heat-stressed nematodes that were incubated for 3 h (lane 1) or 1 h (lane 2) at 31 C. The lower part shows a portion of the same blot that was hybridized with a 32P-labeled cDNA probe for myosin light chain mRNA, as described previously. Levels of the two myosin light chain transcripts do not vary with stress or development (not shown). Thus, the resulting signals document the integrity of the RNA samples and show that equal amounts of RNA were loaded in each lane.
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  Crop: Crop_picture: WBPicture0000008101
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1996
    Article_URL: DOI id 10.1074/jbc.271.47.30158
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00002615