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WBPicture0000008094 Description: Figure 7. Analysis of in situ expression of CePAK, CeRac1, and CDC42Ce by immunofluorescence. Embryos were prepared for immunofluorescence analysis as described. Panel A shows the dorsal view of an embryo at the beginning of body elongation; all the hypodermal cell boundaries are stained by the CePAK antibody. The arrowhead points to one circumferential boundary. Panels B-E represent the staining pattern of embryos undergoing hypodermal fusion and body elongation. Panel B is the lateral view of an embryo where the staining can be seen at both circumferential (indicated by small arrowhead) and longitudinal (indicated by big arrowhead) cell boundaries. Panel C is the lateral view of an embryo at a later stage of the elongation event (compared with that in panel B), where the staining of the circumferential boundary disappears but that of the longitudinal boundary remains clearly visible (arrowhead). Panel D is the lateral view of an embryo at the end of the elongation event. Only the longitudinal boundaries are stained (indicated by two arrowheads) as all circumferential ones have fused into each other. Panel E is the dorsal view of an embryo at the about same stage as that in panel D. The two longitudinal cell boundaries can be seen (indicated by arrowheads). Panel F is the lateral view of an embryo stained by MH 27 monoclonal antibody, which exclusively recognizes the hypodermal cell boundaries. Panel G is the dorsal view of an embryo, which is at the late stage of the body elongation, stained with the affinity-purified anti-CeRac1 antibody (see also panel I). The two longitudinal boundaries (indicated by arrowheads) are visible, similar to the staining pattern of CePAK in panel E. Panel H is the lateral view of an embryo during the elongation event stained with the affinity-purified anti-CDC42Ce antibody (see panel I). Staining of both the circumferential (indicated by small arrowhead) and the longitudinal boundaries (indicated by big arrowhead) were observed. Panel I shows the Western blot analysis with the affinity-purified anti-CeRac1 and anti-CDC42Ce antibodies raised in this study. Conditions were identical as for the analysis of the CePAK antibody (see Fig. 4), except that the cytosolic (lane S) and particulate (lane P) fractions here are prepared from mixed stage populations of C. elegans as described (29). The sizes of the Rainbow protein markers (Amersham Corp.) are indicated on the left side of the filter.
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  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1996
    Article_URL: DOI id 10.1074/jbc.271.42.26362
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00002544