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| WBPicture0000008083 | Description | Figure 5. Immunolocalization of OSM-5 protein and antibody specificity. All panels are lateral views of wild type (B,C,E,F) or daf-19(m86) (D) worms processed in parallel for immunofluorescence using the 12B6 antibody. Anterior is leftwards and dorsal is upwards. (A) A hermaphrodite worm (bottom) and a lateral view of the male tail (top). Box a' indicates the region pictured in C-F. Arrows indicate the position of the amphid cilia. In unblocked samples, immunoreactivity is observed in the rays of the male tail (B) and the ciliated amphid and labial neurons (C). Preincubation of the antibody with 10-fold molar excess of purified OSM-5::GST (E) blocks all immunostaining in the amphid and labial neurons, while preincubation of the antibody with purified GST alone (F) has no effect. (D) In the absence of DAF-19, low levels of OSM-5 protein are observed at the tips of dendrites despite the loss of cilia associated with mutations in daf-19. Scale bars: 5 µm. | |
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| Name | FigE.jpg | ||
| Depict | Expr_pattern | Expr856 | |
| Anatomy | WBbt:0005117 | ||
| WBbt:0005394 | |||
| WBbt:0006801 | |||
| WBbt:0006941 | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2001 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00004603 |
