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| WBPicture0000008060 | Description | Figure 2. CEH-17 is expressed in a limited subset of neurons. (A) Wild-type L1 larvae immunostained with an anti-CEH-17 serum, which reveals CEH-17 expression in four ventral SIA nuclei and a single dorsal ALA nucleus. (B,C) Wild-type larvae harboring a pceh-17::GFP extrachromosomal array. (B) The dorsally located ALA cell body sends two branches (arrows) into the nerve ring. The fluorescence in the lateral amphid neuron (asterisk) is due to ectopic expression of the transgene. (C) One dorsal and one ventral SIA neurons each send an anterior axon (arrowheads) into the nerve ring, which then turn posteriorly and run along the sublateral dorsal and ventral cords (arrows), respectively. (D,E) Double immunostaining of CEH-17+neurons with an anti-CEH-17 serum (red) and of SMBneurons with an anti-GFP serum (green) in an Ex[odr-2::GFP] line (D) and an Ex[flp-12::GFP] line (E). No double staining is observed and the CEH-17+neurons are seen posterior to the SMB neurons,confirming their identification as SIAs. In addition to the four SIAs and the four SMBs, odr-2::GFP expressing neurons in the ventral cord (asterisk in D) and the CEH-17+ALA neuron (asterisk in E) areclearly visible. (F-I) Expression of pceh-17::GFP::3in wild-typeembryos. (F,G) At the comma stage, axons (arrowhead in G) can be seen sprouting from the four, brightly fluorescent, ventral SIA neurons. The SIA sister cells, including DB5 and DA8, can also be seen. (H,I) GFP expression is first seen at the late proliferation stage in eight cells forming three groups (see text for details). In all panels, anterior is to the left. | |
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| Name | FigA.jpg | ||
| Depict | Expr_pattern | Expr1039 | |
| Anatomy | WBbt:0003955 | ||
| WBbt:0004842 | |||
| WBbt:0004858 | |||
| WBbt:0004983 | |||
| WBbt:0004985 | |||
| WBbt:0004987 | |||
| WBbt:0004989 | |||
| Cellular_component | GO:0005634 | ||
| GO:0030424 | |||
| GO:0043025 | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2000 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00004283 |
